| Test
180 - Neisseria gonorrhoeae DNA by PCR |
| Method |
Polymerase
Chain Reaction (PCR) - Qualitative |
| Specimen
Requirements |
ThinPrep(tm)
Solution:1.0 ml (0.5 ml); SurePath(tm): 0.50
ml (0.25 ml), Ambient; Urine: 10.0 ml (5.0 ml),
Refrigerated or FROZEN; NAT(tm) or STD Swabs: from
female endocervix or male urethra (NAT(tm) swab provided
by Genetic Assays).
|
| Collection
& Transport |
ThinPrep(tm)
or SurePath(tm) Solution: Store and ship ThinPrep(tm)
or SurePath(tm) Solution at ambient temperature up to
21 days;
Urine: Collect first catch (not midstream) urine
in sterile, leakproof container. The patient should not
have urinated for 2 hours prior to collection. Immediately
store urine at 2-8°C and ship within 24 hours of collection
on cold pack;
NAT(tm) or STD Swabs: Transport swab at ambient
temperature within 24 hours of collection. |
| Causes
for Rejection |
ThinPrep(tm)
Solution: Less than 0.50 ml and/or more than 21 days;
SurePath(tm): Less than 0.25 ml and/or more than
21 days; Urine: Less than 10.0 ml, and/or more
than 24 hours at non refrigerated temperature (2-8∞C);
NAT(tm) or STD Swabs: Bloody swab or no swab in
transport tube. |
| Specimen
Stability |
ThinPrep(tm)
or AutoCyte(tm) Solution: Stable for up to 21 days
at room temperature; Urine: Stable for up to 7
days at 2-8°C; NAT(tm) or STD Swabs: Stable
for up to 7 days at 2-8°C. |
| Reference
Range |
Not
Detected |
| TAT |
24-48
hours |
| CPT
Code |
87591,
Neisseria gonorrhoeae, amplified probe technique |
|
The
speed and enhanced sensitivity of the Roche amplified
DNA probe methodology enables early, accurate detection
of Neisseria gonorrhoeae, resulting in timely administration
of therapy. Several publications involving multiple
centers have demonstrated a sensitivity of greater than
97% sensitivity in the detection of gonorrhea , , .
The specificity is greater than 99%. Swab samples had
better sensitivity than urine samples for the detection
of both Chlamydia and GC.
|
