| Test
3700 - BK Virus DNA by Real-Time PCR - Quantitation |
| Method |
Real-Time Polymerase
Chain Reaction (PCR) – Viral Load Monitoring |
| Specimen
Requirements |
5.0 ml (1.0ml) Urine*: Refrigerated; 5.0 ml (3.0 ml) EDTA or ACD Whole Blood*: Refrigerated; 2.0 (1.0 ml) Serum: Refrigerated or Frozen; EDTA blood plasma: 5.0 ml (3.0ml), separate plasma from red cells within
6 hours and store at 2-8°C if shipping within 24 hours or store at 20°C if shipping > 24 hours; 5.0 ml (1.0 ml) PPT Plasma: Refrigerated or Frozen;
2.0 ml (1.0ml) CSF: Refrigerated or Frozen.
* Serial testing of urine and blood is recommended. |
| Collection
& Transport |
Urine: collect in sterile container and ship refrigerated; Whole Blood EDTA or ACD: ship refrigerated; EDTA blood plasma: separate plasma from red cells within 6 hours and store at 2-8°C if shipping within 24 hours or store at -20°C if shipping > 24 hours.
CSF, PPT Plasma, Serum: ship refrigerated or frozen. |
| Causes
for Rejection |
Whole
blood: in heparin; Plasma not separated within 6 hours;
All: insufficient volume or shipped at incorrect temperature |
| Specimen
Stability |
Refrigerated specimens stable for seven days; ambient specimens stable for four days; frozen specimens stable indefinitely. |
| Reference
Range |
Less than 500 BKV DNA copies/ml
Assay Results Range: 500 to 1.0 x 10e10 BKV DNA copies/ml. |
| TAT |
24-72
hours |
| CPT
Code |
87799, Infectious agent detection
by nucleic acid, not otherwise specified; quantification
probe technique |
| BK
virus (BKV) DNA quantitation is based upon the real-time
PCR amplification and detection
of BKV genomic DNA. The quantitative range of this
assay is 500 to 1.0 x 10e10 BKV DNA copies/ml. A patient
value of less than 500 BKV DNA copies/ml indicates
that the patient’s viral load is below the quantitative
limit of this assay, and does not indicate that the
patient is not infected with BKV.
|
