5.0 ml (1.0 ml) Amniotic fluid: discard first 2 ml, then collect 3-5 ml in a sterile plastic conical tube or one T-25 flask of confluent cells;  2.0 ml (1.0ml) CSF: Refrigerated or Frozen;  5.0 ml (3.0 ml) EDTA or ACD Whole Blood: Refrigerated;  5.0 ml (1.0ml) Urine:Refrigerated; 2.0 (1.0 ml) Serum:Refrigerated or Frozen; 5.0 ml (1.0 ml) PPT Plasma: Refrigerated or Frozen; 5.0 ml (1.0ml) EDTA blood plasma: Refrigerated or Frozen.

Amniotic fluid: transport at ambient temperature up to 24 hrs or refrigerate (2-8°C) up to 7 days;  CSF,PPT Plasma, Serum:  ship refrigerated or frozen;  Whole Blood EDTA or ACD: ship refrigerated; Urine: collect in sterile container and ship refrigerated; EDTA blood plasma: separate plasma from red cells within 6 hours and store at 2-8°C if shipping within 24 hours or store at -20°C if shipping > 24 hours.

Whole blood: in heparin; EDTA blood plasma not separated within 6 hours; 
All:  insufficient volume or shipped at incorrect temperature.

Refrigerated specimens stable for 7 days; ambient specimens stable for 4 days;  frozen specimens stable indefinitely.

Adenovirus DNA by Real-time PCR quantitation is a sensitive and reliable method to follow the viral load, and is based upon the real-time PCR amplification and detection of Adenovirus genomic DNA.  The quantitative range of this assay is 100 to 1 x 1010 Adenovirus DNA copies/ml.  A patient value of less than 100 Adenovirus copies/ml indicates that the patients viral load is below the quantitative limit of this assay and does not indicate that the patient is not infected with Adenovirus.