| Test
3702 - Cytomegalovirus (CMV) DNA by Real-Time PCR
- Quantitation |
| Method |
Real-Time Polymerase
Chain Reaction (PCR) – Viral Load Monitoring |
| Specimen
Requirements |
5.0
ml (1.0 ml) Amniotic fluid: discard first 2 ml, then
collect 3-5 ml in a sterile plastic conical tube or
one T-25 flask of confluent cells; 2.0 ml (1.0ml) CSF:
Refrigerated or Frozen; 5.0
ml (3.0 ml) EDTA or ACD Whole Blood: Ambient; 10.0 ml
(5.0ml) Urine:Refrigerated; 2.0 (1.0 ml) Serum:Refrigerated
or Frozen; 5.0 ml (1.0 ml) PPT Plasma: Refrigerated or
Frozen; 5.0 ml (1.0ml) EDTA blood plasma: Refrigerated
or Frozen.
|
| Collection
& Transport |
Amniotic
fluid: transport at ambient temperature up
to 24 hrs or refrigerate (2-8∞ deg. C) up to
7 days; CSF,
Urine, PPT Plasma, Serum: ship refrigerated
or frozen; Whole
Blood EDTA or ACD:
ship ambient; EDTA blood plasma: separate
plasma from red cells within 6 hours and store at 2-8∞C
if shipping within 24 hours or store at 20 deg. C
if shipping > 24
hours. |
| Causes
for Rejection |
Whole
blood: in heparin; EDTA blood plasma not separated within
6 hours;
All: insufficient volume or shipped at incorrect temperature. |
| Specimen
Stability |
Refrigerated and ambient specimens
stable for four days; frozen specimens stable indefinitely. |
| Reference
Range |
Less than 100 CMV DNA copies/ml
Assay Results Range: 100 to 1 x 10e10 CMV DNA copies/ml. |
| TAT |
24-72
hours |
| CPT
Code |
87497, Quantification probe
technique |
|
Cytomegalovirus is a commonly found virus that threatens
immunocompromised patients including neonates, transplant
recipients, oncology patients or patients with AIDS.
CMV is the leading viral opportunistic infection in
persons with AIDS and the most common cause of congenital
infection in the developed world . Commonly seen manifestations
of a CMV infection include: Encephalitis, retinitis,
colitis, hepatitis, adrenalitis, polyradiculopathy
and esophagitis. The amplification of human CMV DNA
by PCR offers very sensitive and specific isolation
and detection of the virus from a variety of clinical
specimen types .
|
